Molecular Epidemiology and Phylogenetic Analysis of Peste des Petits Ruminants Virus Circulating in Sheep in Bangladesh

Peste des petits ruminants (PPR) is a viral disease of small that highly contagious, severe, reportable, and economically important. The present study was conducted to detect the PPR virus (PPRV) circulating in sheep Bangladesh determine its association with epidemiological risk factors degree relationship between F H genes PPRV those other goat isolates. A cross-sectional five selected districts collect data on locations, ecological zones, breeds, age, sex, sources, time period, farming systems using structured questionnaire accompanied by face-to-face interviews. During sampling, 250 nasal swab samples were collected from live typical clinical signs PPR. Thereafter, reverse-transcriptase polymerase chain reaction (RT-PCR) assay employed genes. Risk determined bivariable multivariable logistic regression analyses. Phylogenetic analysis detected performed MEGA software after sequencing both Using RT-PCR, 35.6% (89/250, 95% CI: 29.6%–41.6%) found be positive for PPRV. Locations, feeding identified as potential molecular infection multivariate model. Nucleotide phylogenetic showed strain genetically related lineage IV For gene, sequence divergence our gene ranged 0.01% 0.018% within IV, similarity 98.2% 99.0%. In case similar results also observed divergence, ranging 0.017% 0.083% among others, varied 91.7% 98.3%. To best knowledge, this first RT-PCR-based epidemiology sheep. This highlights importance establishing successful interventions managing infections Bangladesh.